Day 1: (Monday, April 22). Label 4 microfuge tubes. Add 5 microliters of one of the DNA samples (crime scene, suspect 1, suspect 2, suspect 3). Add 5 microliters of restriction enzyme (EcoR1) into each of the tubes. Restriction Enzyme must be kept on ice! Put your tubes in your styrofoam "floatie" and put in the water bath for an hour! After an hour, remove your labeled tubes and put them in the designated rack, which will be refrigerated until Wednesday.
Day 2 (Wed, April 24): Take your samples from Monday and warm them up in your hands. Add 2 microliters of dye to each of the 4 samples and spin in the centrifuge. Carefully load 10 microliters of each of the 4 samples into a gel. Make sure that you make a key so that you know which sample is where. After both groups have loaded their samples into the gel, one group needs to load the "ladder" in one of the leftover lanes in the middle. The gels will be hooked up to the power source and turned on. You can watch bubbles form and then watch the blue dye migrate across the gel. You should label the baggy that your gel was in with your groups' names and then put it near your gel. This will ensure that I will put your gel in your labeled baggy after the gels are done running (because they will be done after you leave).
Day 3 (Fri, April 26). You will get the baggy with your gel. Go into the microscope room with your gel and put it in the "illuminator". Turn the lights off, turn the illuminator on, and view your gel! Have a few different people take photos of the gel. When you leave the room, determine which photo works best and share it with your group numbers. You can also send it to me!
Write up details to follow.
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