April 24-May 5

• Tues 4/25:  Finish notes on Enzymes.  Start Notes on Biotechnology:  stem cells and PCR.  Debrief Enzyme Lab and work on finishing write-up.  First draft due on Thursday 4/27.  Bring a hard copy of your lab or an electric version that another student can evaluate.
• Thurs 4/27:  Peer Review of Enzyme Lab.  Gel electrophorosis and DNA profiling.   Final Enzyme Lab due next Wed 5/3 through turnitin.com
• Mon 5/1:  Finish genetic modification.  Clones!  Do DBQ on p. 195 in Notebook and go to Boston Children's Hospital stem cell site and describe 5 pathways to making different types of stem cells.  Include what those stem cells are used for.
• Wed 5/3:  Turn in Enzyme Lab through turnitin.com.  Biotechnology ethics.
• Fri 5/5:  Finish Biotechnology ethics and practice micropipetting.

What's On The Replication, Transcription, and Translation Test?

First of all, check out the review sheet in the documents section of the blog.  Read through the statements and make sure that you are familiar with the terms and have the skills.  If you have any questions about it, ask in class or shoot me an e-mail.

Now for the format and specific topics for the test:

• 15-20 multiple choice questions.  Pay attention during the Kahoot review to get an idea of what the questions will be like
• Labeling a replication fork with leading strand, lagging strand, helicase, primer, direction of synthesis, etc.  
• You should also be able to outline the process of replication, using names of the enzymes and correct terminology
• Be able to outline the steps of transcription
• Be able to outline the structure of a nucleosome and its importance in controlling gene expression, including how they are modified by epigenetics (methyl and acetyl markers)
• Be able to outline the process of translation, including assembly of the translation initiation complex, the multiple repeated steps in the process of elongation, and termination
• Be able to write the RNA sequence for a given polypeptide and write the sequence for a polypeptide when given the RNA sequence (I will provide you with the genetic code table, but you need to know how to read it).
• Additionally, I will ask you to add or subtract nucleotides from an RNA sequence and write the resulting RNA sequence and amino acid sequence. 

What's Coming Up?

Welcome Back After Spring Break!  I hope it was restful for you!  Here is what we have coming up:

• Tues 4/4:  Finish checking off DBQ's and Epigenetics Video worksheet.  Finish Transcription Notes.  Translation.  Work on Genetic Code Worksheet.  
• Thurs 4/6:  Finish genetics code worksheet and get checked off.  Review Replication, Transcription, and Translation for test to be taken next Tuesday 4/11.
• Fri 4/7:  Full 8 Day.  Short Classes (43 minutes):  Play Kahoot to Review Replication, Transcription, and Translation.
• Tues 4/11:  Replication, Transcription, and Translation Test.  Start Enzyme Worksheet.   Read 2.5. 
• Thurs 4/13:  Finish Enzyme Worksheet.  Start Enzyme Lab.  Read 8.1.
• Mon 4/17:  Enzyme Lab:  finish protocols and brainstorm what data you will take for the real lab.
• Wed 4/19:  Figure out the quantities that you will use for your lab and do the pre-write for your lab (Background, focused question, variables, materials, procedure, justification for choices made.
• Fri 4/21:  Take data for the lab and start processing the data.  Discuss final lab write-up.  First complete draft due Thursday 4/27.  Final Draft due Wed 5/3.  Start reading about biotech (3.5).