4/27-4/30

• Mon 4/27:  Restriction Enzyme Worksheet.  Work on Bioethics Posters.
• Tues 4/28:  1st period doesn't have class due to ACT.  3rd and 4th periods will finish posters, turn in, and set up Seed Germination.
• Thurs 4/30:  Questions before Biotech Quiz?  Take Quiz.  1st period does set up for Seed Germination and then beginning notes on plants.  3rd and 4th periods takes quiz and then does Leaf Structure coloring and worksheet.

Biotechnology Quiz on Thursday 4/30

Syllabus Statements for Biotech:

• Gel electrophoresis is used to separate proteins or fragments of DNA according to size
• PCR can be used to amplify small amount of DNA
• DNA profiling involves comparison of DNA
• Genetic modification is carried out lby gene transfer between species
• Clones are groups of genetically identical organisms, derived from a single original parent cell
• Many plant species and some animal species have natural methods of cloning
• Animals can be cloned at the embryo stage by breaking up the embryo into more than one group of cells
• Methods have been developed for cloning adult animals using differentiated cells

Applications and skills:

• Be able to describe the use of DNA profiling in paternity and forensic investigations
• Be able to describe the process of gene transfer to bacteria using plasmids, restriction endonucleases, and DNA ligase
• Be able to assess potential risks and benefits associated with genetic modification of crops
• Be able to describe the process of making cloned embryos produced by somatic-cell nuclear transfer (as in Dolly the sheep)

The Week of 4/20-4/24

• Mon 4/20:  Turn in Stem Cell Interactives if not already.  Using new IB books in the classroom, take notes on Gel Electrophoresis, PCR, and DNA profiling.  Do PCR and Gel Electrophoresis simulations on the computers. SPARK lecture today!!
• Tues 4/21:  Watch videos on application of stem cells:  making artificial organs and skin cell spray guns.  Now, using the new IB books, take notes on gene transfers to bacteria and risks and benefits of Genetically Modified crops.  Do data-analysis questions on Analyzing risks to Monarch butterflies of Bt corn (p. 195) on separate paper to be turned in. If time, continue to bacterial transformation simulation on-line.
• Thurs 4/23:  Review gel electrophoresis, PCR, and bacterial transformation.  Research issues of genetic modification and make a poster.

4/13-4/17

Welcome to the 4th Quarter!!

Mon 4/13:  Go over enzyme lab write-up and concepts on Enzyme Quiz.  Work time.
Tues 4/14:  Take Enzyme Quiz.  Time to either work on writing up the Enzyme Lab (including LoggerPro hel0) or to do the 2 Stem Cell On-line Interactives.  Instructions below.
Thurs 4/16:  Turn in Enzyme Lab (hard-copy and through turnitin.com).  Notes on Stem Cells and Cloning (see documents section if you need them).  More time to work on Stem Cell interactives.  Turn those in today or Monday.

Enzyme Lab due Thursday 4/16 through turnitin.com and a hard copy

What do you need?
1)  Focused Question
2)  Background information
3)  Variables
4)  Materials
5)  Procedure
6)  Data Table
7)  Calculations
8)  Graph (s)
9)  Conclusion
10)  Evaluation

Click here for help on the Data Parts, Conclusion, and Evaluation.

Virtual Stem Cell Laboratory Assignment and Go Go Stem Cell!

Go to On-Line Virtual Stem Cell Laboratory and Do/Answer the Following on Separate sheet of paper.  You may turn in one sheet for two people.

1)  Define stem cell
2)  What are embryonic stem cells?
3)  Try the coaxing agents and see what they do.  Trace at least 4 pathways to their endpoints.  Describe 4 of these pathways.
4)  Describe what these 4 endpoints are used for (read the text on the website).  For example, motor neuron cells can be used to treat ALS!

Go to Learn Genetics Link and go to Go Go Stem Cell!
1)  What is a niche?
2)  Go to 3 of the possible stem cell niches and go through the simulation. Describe what happens to the cell as it is converted from a stem cell to its particular differentiated cell.

Topics for Enzyme Quiz on Tues 4/14

You can use the Enzyme Notes on this site to help access this information.

1. Define enzyme and active site
2. Explain enzyme-substrate specificity (lock and key)
3. Explain the effects of temperature, pH, and substrate concentration on the enzyme activity (and be able to identify and/or sketch the graphs)
4. Define enzyme denaturation and describe ways in which it occurs
5. State that metabolic pathways consist of chains and cycles of enzyme-catalyzed reactions.
6. Describe the induced-fit model for enzymes (extension of lock and key model)
7. Explain that enzymes lower the activation energy of the chemical reactions that they catalyze (and be able to identify this effect on a graph)
8. Explain the difference between competitive and non-competitive inhibition, with reference to one example of each.
9. Explain the control of metabolic pathways by end-product inhibition, including the role of allosteric sites.

End of 3rd Quarter!!

SPARK Lecture  on Fisheries on Monday!!

• Mon 4/6:  Do 2 other protocols for Enzyme Lab and fill out land hand-out notes.  Please decide what protocol you want to do and what you will change and turn in to me before you go today.
• Tues 4/7:  Do trials for the lab to determine which quantities you will use.  Lab pre-write and start actual trials if you have time.
• Thurs 4/9:  Complete trials for the lab and start processing data.  Completed Write-up is due next Thursday 4/16.  Review for short Enzyme, Protein, and DNA Test next Tuesday 4/14.  All end of the quarter work due before the end of the school day.
• Friday 4/10:  No School!  Teacher Work Day.